Light is an important factor affecting the growth of in vitroexplant. Multiple cultures will increase costs and cause the variation of genes. In this study, effects of Yellow (Y), Red (R), Blue (B), 50%Y: 50%B, 50%Y: 50%R, 70%Y: 30%B, and 70%Y: 30%R were evaluated. Study results showed the light of 70%Y: 30%R effectively inhibited the growth of NC 628, and the plant quality was equivalent to W. With Burley 64, lights were unsuitable because they caused the ageing quickly. The 70%Y: 30%R light effectively inhibited the growth of 05 genetic resources, and plant quality was equivalent to W at the 100 DAC period. Next to 170 DAC, 70%Y: 30%R light inhibited the growth compared to the W, but caused the ageing of genetic resources faster than W. Light of Y and R effectively increased the leaf number and plant height, and B light sharply limited the growth of explants. Plant dry weight of 70%Y: 30%R and Y light was highest (0.47 - 0.5g), and lowest was B light (0.2g). Under Y, the leaf size was larger than other lights (1.9x1.99 cm), and the smallest leaf size belonged to B (2.85x1.73cm). Next time, we will research mixed light ratios to determine the suitable light for genetic resources.